Zero-mode waveguide detectors
a | b | c | d | e | f | g | h | i | j | k | l | m | n | o | p | q | r | s | t | u | v | w | x | y | z | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9
Click one of the letters above to advance the page to terms beginning with that letter.
- Zero-mode waveguide detectors search for term
This nanostructure device is 100 nm in diameter, which is smaller than the 532 nm and 643 nm laser wavelengths used in the Pacific Biosciences platform. Light cannot propagate through these small waveguides, hence the term zero-mode. These aluminium-clad waveguides are designed to produce an evanescent wave that substantially reduces the observation volume at the surface of the polymerase reaction down to the zeptolitre range. This provides an advantage for the polymerization reaction, which can be performed at higher dye-labelled nucleotide concentrations. (Metzker 2010)
