Sanger sequencing technology (also known as dideoxy sequencing or chain termination method)
- Sanger sequencing technology (also known as dideoxy sequencing or chain termination method)search for term
used to sequence the first plant genomes following a BAC-by-BAC approach. It is based on the sequencing of DNA fragments amplified after cloning in bacterial vectors and relies on the use of dideoxynucleotides (ddNTPs) in addition to the normal nucleotides (dNTPs) found in DNA. Dideoxynucleotides are the same as nucleotides except that they contain a hydrogen group on the 30 carbon instead of a hydroxyl group (OH). These modified nucleotides, when integrated into a sequence, prevent the addition of further nucleotides and, thus, the DNA chain is terminated. The incorporation of ddNTPs labeled with different fluorescent dyes enables the detection of all terminated sequence products through electrophoresis based on size separation. (Feuillet 2011)
