Reference genome sequences
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ideally referred to as genome-wide sequence data obtained by BAC-by-BAC clone sequencing and/or whole genome shotgun sequencing. These sequences give the physical framework of the genome of a particular individual. In cases for which prior genomic information is not available for the species being studied, transcript sequence data (transcript assembly) or BAC-end sequence data or genome sequences of phylogenetically related species can be considered as the reference genome for analyzing NGS data. (Varshney 2009)