Reduced representation bisulphite sequencing
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- Reduced representation bisulphite sequencing search for term
This technique cuts genomic DNA with restriction enzymes to enrich for CG-rich regions, which are then converted through bisulphite treatment and sequenced with next-generation sequencing. Bisulphite treatment converts unmethylated C to uracil which appears as T in sequencing reads while leaving methylated C intact. (Hawkins 2010)
