Automated Sanger Sequencing
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- Automated Sanger Sequencing search for term
This process for DNA sequencing involves a mixture of techniques: bacterial cloning or PCR; template purification; labelling of DNA fragments using the chain termination method with energy transfer; dye-labelled dideoxynucleotides and a DNA polymerase; capillary electrophoresis; and fluorescence detection that provides four-colour plots to reveal the DNA sequence. (Metzker 2010)
