Adjacent valid colour
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A nucleotide substitution will have two colour calls, one from the 5' position and one from the 3' position of the dinucleotide sequence. When compared with a reference genome, base substitution in the target sequence is encoded by two specific, adjacent colours. For example, the sequence CCT is encoded as blue-yellow (CC = blue; CT = yellow), but substituting the middle C for A would result in two colour changes to green-red. Any other colour sequence can be discarded as an error. (Metzker 2010)