Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae).
| Title | Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae). |
| Publication Type | Journal Article |
| Year of Publication | 2010 |
| Authors | Robert S, Rieux A, Argout X, Carlier J, Zapater M-F |
| Journal | American journal of botany |
| Volume | 97 |
| Pagination | e130-2 |
| Date Published | 2010 Dec |
| ISSN | 0002-9122 |
| Abstract | UNLABELLED: • PREMISE OF THE STUDY: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. • METHODS AND RESULTS: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. • CONCLUSIONS: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations. |
| DOI | 10.3732/ajb.1000295 |
| Alternate Journal | Am. J. Bot. |
