@article {73713,
	title = {Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae).},
	journal = {American journal of botany},
	volume = {97},
	year = {2010},
	month = {2010 Dec},
	pages = {e130-2},
	abstract = {UNLABELLED: {\textbullet}

PREMISE OF THE STUDY: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. {\textbullet}

METHODS AND RESULTS: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. {\textbullet}

CONCLUSIONS: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations.},
	issn = {0002-9122},
	doi = {10.3732/ajb.1000295},
	author = {Robert, St{\'e}phanie and Rieux, Adrien and Xavier Argout and Carlier, Jean and Zapater, Marie-Fran{\c c}oise}
}
