@article {85,
	title = {EU-OSTID: a collection of transposon insertional mutants for functional genomics in rice},
	journal = {Plant molecular biology},
	volume = {59},
	year = {2005},
	month = {2005/09//},
	pages = {99 - 110},
	abstract = {A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for reverse genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59\% of the inserts in genes annotated on the rice chromosomes and 41\% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.},
	isbn = {01674412},
	author = {L J G van Enckevort and Droc, Ga{\"e}tan and Pietro Piffanelli and Raffaella Greco and Cyril Gagneur and Christele Weber and V{\'\i}ctor M Gonz{\'a}lez and Pere Cabot and Fabio Fornara and Stefano Berri and Berta Miro and Ping Lan and Marta Rafel and Teresa Capell and Pere Puigdom{\`e}nech and Pieter B F Ouwerkerk and Annemarie H Meijer and Enrico Pe{\textquoteright} and Lucia Colombo and Paul Christou and Guiderdoni, Emmanuel and Andy Pereira}
}
