@article {73695,
	title = {Sequence and Expression Analyses of Ethylene Response Factors Highly Expressed in Latex Cells from Hevea brasiliensis},
	journal = {PLoS ONE},
	volume = {9},
	year = {2014},
	month = {2014/06/27},
	pages = {e99367 - },
	publisher = {Public Library of Science},
	abstract = {<p>The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In <italic>Hevea brasiliensis</italic>, <italic>ERF</italic> genes have been identified by RNA sequencing. This study set out to validate the number of <italic>HbERF</italic> genes, and identify ERF genes involved in the regulation of latex cell metabolism. A comprehensive <italic>Hevea</italic> transcriptome was improved using additional RNA reads from reproductive tissues. Newly assembled contigs were annotated in the Gene Ontology database and were assigned to 3 main categories. The AP2/ERF superfamily is the third most represented compared with other transcription factor families. A comparison with genomic scaffolds led to an estimation of 114 <italic>AP2/ERF</italic> genes and 1 soloist in <italic>Hevea brasiliensis</italic>. Based on a phylogenetic analysis, functions were predicted for 26 <italic>HbERF</italic> genes. A relative transcript abundance analysis was performed by real-time RT-PCR in various tissues. Transcripts of ERFs from group I and VIII were very abundant in all tissues while those of group VII were highly accumulated in latex cells. Seven of the thirty-five ERF expression marker genes were highly expressed in latex. Subcellular localization and transactivation analyses suggested that <italic>HbERF-VII</italic> candidate genes encoded functional transcription factors.</p>},
	url = {http://dx.doi.org/10.1371\%2Fjournal.pone.0099367},
	author = {Piyatrakul, Piyanuch and Yang, Meng and Putranto, Riza-Arief and Pirrello, Julien and Dessailly, Florence and Hu, Songnian and Summo, Marilyne and Theeravatanasuk, Kannikar and Leclercq, Julie and Kuswanhadi, and Montoro, Pascal}
}
