Total internal reflection fluorescence
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A total internal reflection fluorescence imaging device produces an evanescent wave that is, a near-field stationary excitation wave with an intensity that decreases exponentially away from the surface. This wave propagates across a boundary surface, such as a glass slide, resulting in the excitation of fluorescent molecules near (<200 nm) or at the surface and the subsequent collection of their emission signals by a detector. (Metzker 2010)
