Fluorescence resonance energy transfer (FRET) 


Fluorescence resonance energy transfer (FRET) search for term

This is generally a system that consists of two fluorescent dyes, one being a donor dye (a bluer fluorophore) and the other an acceptor dye (a redder fluorophore). When the two dye molecules are brought into close proximity (usually 30 nm), the energy from the excited donor dye is transferred to the acceptor dye, increasing its emission intensity signal. (Metzker 2010)