%0 Journal Article %J American journal of botany %D 2010 %T Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae). %A Robert, Stéphanie %A Rieux, Adrien %A Xavier Argout %A Carlier, Jean %A Zapater, Marie-Françoise %N 12 %P e130-2 %R 10.3732/ajb.1000295 %V 97 %X UNLABELLED: • PREMISE OF THE STUDY: Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. • METHODS AND RESULTS: Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. • CONCLUSIONS: The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations. %8 2010 Dec